Effects of Exogenous Glycinebetaine on Growth and Ultrastructure of Salt-Stressed Rice Seedlings (Oryza sativa L.)

By Shahidur Rahman, H. Miyake, Y. Takeoka. 2002

141 citations recorded by the source corpus.

Abstract The effects of exogenously applied glycinebetaine on the salt-stress-induced inhibition of growth and ultrastructural damages in rice seedlings were investigated. Glycinebetaine was not effective in alleviating the NaCl-induced inhibition of root growth and rather enhanced the NaCl-induced inhibition. However, it was found to alleviate the inhibition of shoot growth induced by NaCl stress. Concentrations of Na were higher in salt-stressed plants than in unstressed plants. Stressed plants receiving glycinebetaine had a significantly lower Na and higher K concentrations in the shoots than the plants grown without application of glycinebetaine. Salinity induced ultrastructural damages in leaf such as swelling of thylakoids, disintegration of grana stacking and intergranal lamellae and destruction of mitochondria (deficiency of cristae, swelling and vacuolation). Such damages were largely prevented by pretreatment with glycinebetaine resulting in greening of the plants. In roots, the epidermis, cortex and root cap were more sensitive to salt stress than the meristem and stele. The most frequently observed ultrastructural alteration due to NaCl salinity was the formation of many large vacuoles in the root tip and root cap cells. The number of mitochondria was increased and they were aggregated in the cytoplasm of the root tip and root cap cells by treatment with NaCl or NaCl plus glycinebetaine. Glycinebetaine could not prevent the NaCl-induced ultrastructural damages in root cells. The effects of glycinebetaine to mitigate the ultrastructural damages in the chloroplast and mitochondria induced by NaCl might be due to the production of many vacuoles in root cells which may act to store Na and decrease its accumulation in the shoot.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/41e0af2e52298de4fdd389505a6f5bb5264438f2

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In Plant Science

1. Kamrun Nahar
2. M. Hasanuzzaman
3. M. Fujita
4. A. Wahid
5. S. Cha-um
6. C. Kirdmanee
7. M. R. Mohamed
8. M. Turan

Citations in the corpus

• Plant Response to Salt Stress and Role of Exogenous Protectants to Mitigate Salt-Induced Damages (2013, background): “Rahman et al. ( 2002 ) reported the bene fi cial effect of GB on the ultrastructure of salt-stressed O. sativa seedlings.”
• Effect of glycinebetaine on proline, water use, and photosynthetic efficiencies, and growth of rice seedlings under salt stress (2010, background): “There are many reports on improving water relations, maintaining pigments, and improving photosynthetic abilities and growth by using exogenous Glybet treatments for salt tolerance in rice (Rahman et al. 2002; Demiral and Türkan 2004; Chaum et al. 2006; Demiral and Türkan 2006), wheat (Raza et al.”
• Roles of glycine betaine in mitigating deleterious effect of salt stress on lettuce (Lactuca sativa L.) (2015, result): “These results are in accordance with earlier results in which GB treatments improved the uptake of nutrient elements but decreased the accumulation of Na (Mäkela et al. 1999; Rahman et al. 2002; Abbas et al. 2010; Habib et al. 2012).”
• Glycine-betaine induced salinity tolerance in maize by regulating the physiological attributes, antioxidant defense system and ionic homeostasis (2021, background): “Moreover, the GB differentiates Na against K under SS conditions (Mansour 1998), and improves the root cells' efficacy to accumulate more Na in plants grown under SS conditions (Rahman et al., 2002).”
• Overexpression of the betaine aldehyde dehydrogenase gene from Atriplex hortensis enhances salt tolerance in the transgenic trifoliate orange (Poncirus trifoliata L. Raf.) (2011, result): “This is in agreement with earlier tudies reporting that an increased GB level was correlated with educed accumulation of Na+ and increased levels of K+ upon expoure to salt stress (Rahman et al., 2002; Raza et al., 2007; Zhou et al., 007; He et al., 2010).”
• Salt stress and phyto-biochemical responses of plants - a review (2018, background): “…of exogenous application of GB on salt-induced shoot- and root growth inhibition and ultra-structural damages were studied in rice seedlings (Rahman et al. 2002), however, there are a few reports suggesting a lack of such positive effects or even apparent negative effects of exogenous GB on…”
• Induction of Heat Stress Tolerance in Barley Seedlings by Pre-Sowing Seed Treatment with Glycinebetaine (2005, background): “Nevertheless, external use of GB was found to protect rice and sugarcane against salinity (Rahman et al. 2002; Wahid 2004), wheat against drought (Wen-Yue et al. 2001), Arabidopsis against freezing stress (Xing and Rajashekar 2001) and to confer osmotic adaptation upon the prokaryotic and…”
• Effect of selenium and silicon on transcription factors NAC5 and DREB2A involved in drought-responsive gene expression in rice (2014, background): “Glycine betaine is an inert molecule and protects the oxygen-evolving photosystem II complex and ATP synthesis in onion and rice (Mansour 1998, Rahman et al. 2002).”
• Mechanism of Salinity Tolerance in Plants: Physiological, Biochemical, and Molecular Characterization (2014, background): “The main aim of this review is to discuss research advances on the complex physiological and molecular mechanisms that are involved in plant salinity tolerance.”