Linking alkaline phosphatase activity with bacterial phoD gene abundance in soil from a long-term management trial
By Tandra D. Fraser, D. Lynch, M. Entz, K. Dunfield. 2015
234 citations recorded by the source corpus.
Changes in land management practices may have significant implications for soil microbial communities important in organic P turnover. Soil bacteria can increase plant P availability by excreting phosphatase enzymes which catalyze the hydrolysis of ester-phosphate bonds. Examining the diversity and abundance of alkaline phosphatase gene harboring bacteria may provide valuable insight into alkaline phosphatase production in soils. This study examined the effect of 20 years of no input organic (ORG), organic with composted manure (ORG + M), conventional (CONV) and restored prairie (PRA) management on soil P bioavailability, alkaline phosphatase activity (ALP), and abundance and diversity of ALP gene (phoD) harboring bacteria in soils from the northern Great Plains of Canada. Management system influenced bioavailable P (P < 0.001), but not total P, with the lowest concentrations in the ORG systems and the highest in PRA. Higher rates of ALP were observed in the ORG and ORG + M treatments with a significant negative correlation between bioavailable P and ALP in 2011 (r2 = 0.71; P = 0.03) and 2012 (r2 = 0.51; P = 0.02), suggesting that ALP activity increased under P limiting conditions. The phoD gene abundance was also highest in ORG and ORG + M resulting in a significant positive relationship between bacterial phoD abundance and ALP activity (r2 = 0.71; P = 0.009). Analysis of phoD bacterial community fingerprints showed a higher number of species in CONV compared to ORG and ORG + M, contrary to what was expected considering greater ALP activity under ORG management. In 2012, banding profiles of ORG + M showed fewer phoD bacterial species following the second manure application, although ALP activity is higher than in 2011. This indicates that a few species may be producing more ALP and that quantitative gene analysis was a better indicator of activity than the number of species present.
Subfields: Environmental Chemistry, Plant Science, Soil Science
View Paper: https://www.semanticscholar.org/paper/41d87e627d93770786b4cf23015f26c1c15f7b89
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In Environmental Chemistry
In Plant Science
In Soil Science
Citations in the corpus
- Soil bacterial phoD gene abundance and expression in response to applied phosphorus and long-term management (2015, methodology): “The organic field sample with a manure application demonstrated a shift in the phoD bacterial community, but not gene abundance, to bemore similar to the conventional managed system than the organic no input system (Fraser et al., 2014).”
- Total and active microbial communities and phoD as affected by phosphate depletion and pH in soil (2016, background): “In a long-term trial including non-fertilized soils, soil fertilized with composted manure or water-soluble phosphate, and prairie soils, Fraser et al. (2015) observed a general positive correlation between phoD gene abundance and potential ALP activity in soil, suggesting that PhoD contributes…”
- Quantification of bacterial non-specific acid (phoC) and alkaline (phoD) phosphatase genes in bulk and rhizosphere soil from organically managed soybean fields (2017, methodology): “Some experiments have demonstrated increased phosphatase activity under phosphate-limited conditions (Fraser et al., 2015a,b; Kier et al., 1979; Zhang et al., 2012), although others have found no correlation (Fraser et al., 2015b).”
- Long-term fertilisation regimes affect the composition of the alkaline phosphomonoesterase encoding microbial community of a vertisol and its derivative soil fractions (2017, background): “It has been known already that the phoD gene abundances and ALP activity are significantly correlated in soil under different management systems (Fraser et al. 2015b), and that the variation in the composition of the phoD gene induced by fertilisation regimes can affect ALP activity (Tan et al.…”