Estimating cell numbers

By P. Andersen, J. Throndsen. 2003

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First record of a fish-killing Gymnodinium sp. bloom in Kuwait Bay, Arabian Sea: chronology and potential causes (2001, methodology): “Samples were mixed by gently inverting the sample container twice, then duplicate 0.1 ml volumes from each sample were counted using a Palmer-Maloney counting chamber and an Olympus BH-2 microscope according to Throndsen (1995).”
Sampling harmful benthic dinoflagellates: Comparison of artificial and natural substrate methods (2014, unknown): “The piece of nylon mesh was then transferred to a 15 ml screw-cap tube containing 5–10 ml of GF/ F (Whatman(1), Piscataway, NJ, USA) filtered seawater and the sample was preserved with 1–2 drops of neutral Lugol’s iodine solution or 1% glutaraldehyde (Throndsen, 1995).”
PRODUCTION OF PARALYTIC SHELLFISH TOXINS BY APHANIZOMENON SP. LMECYA 31 (CYANOBACTERIA)1 (2002, methodology): “Cell density was measured by observation of Lugol’s preserved aliquots under the inverted microscope, using either Sedgewick-Rafter or Palmer-Maloney chambers (Throndsen 1995).”
Bipolar distribution of the cyst-forming dinoflagellate Polarella glacialis (2003, methodology): “A unialgal culture was subsequently obtained in the laboratory using the serial dilution method (Throndsen 1995).”