A herpesvirus protein selectively inhibits cellular mRNA nuclear export
By D. Gong, Yong Hoon Kim, Yuchen Xiao, Yushen Du, Yafang Xie, K. K. Lee, Jun Feng, Nisar Farhat, Dawei Zhao, S. Shu and 6 more. 2016
52 citations recorded by the source corpus.
SUMMARY Nuclear mRNA export is highly regulated to ensure accurate cellular gene expression. Viral inhibition of cellular mRNA export can enhance viral access to the cellular translation machinery and prevent anti-viral protein production but is generally thought to be nonselective. We report that ORF10 of Kaposi's sarcoma associated herpesvirus (KSHV), a nuclear DNA virus, inhibits mRNA export in a transcript-selective manner to control cellular gene expression. Nuclear export inhibition by ORF10 requires an interaction with an RNA export factor, Rae1. Genome-wide analysis reveals a subset of cellular mRNAs whose nuclear export is blocked by ORF10 with the 3' untranslated regions (3' UTRs) of ORF10-targeted transcripts conferring sensitivity to export inhibition. The Rae1-ORF10 interaction is important for the virus to express viral genes and produce infectious virions. These results suggest that a nuclear DNA virus can selectively interfere with RNA export to restrict host gene expression for optimal replication.
Subfields: Epidemiology, Infectious Diseases, Oncology
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Citations in the corpus
- Structural basis for Sarbecovirus ORF6 mediated blockage of nucleocytoplasmic transport (2022, methodology): “100 ng RNA were subjected to Real- Nature Communications | (2022) 13:4782 9 time PCR (Quantstudio 7, Applied Biosystems) to determine the GFP RNA transcript copy number, and the primers for RT-PCR were designed according to Gong et al.20.”
- SARS-CoV-2 ORF6 disrupts nucleocytoplasmic transport through interactions with Rae1 and Nup98 (2020, background): “interactions with the nuclear mRNA export factor Rae1 and nuclear pore complex 156 component Nup98 (6, 8).”
- Molecular mechanism underlying selective inhibition of mRNA nuclear export by herpesvirus protein ORF10 (2020, methodology): “We employed a previously reported GFP expression plasmid as a reporter, which is sensitive to ORF10-mediated inhibition and carries the cytomegalovirus promoter and the simian virus 40 (SV40) early polyadenylation signal (25).”
- SARS-CoV-2 ORF6 Disrupts Bidirectional Nucleocytoplasmic Transport through Interactions with Rae1 and Nup98 (2021, background): “VSV M and KSHV ORF10, which both interact with Rae1 and Nup98, produce an accumulation of mRNA in the nuclei of transfected cells (7, 9).”