Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum

By Jing Wang, J. Qian, Ling-Yun Yao, Yanning Lu. Bioresources and Bioprocessing, 2015

161 citations recorded by the source corpus.

BackgroundFlavonoids of Hypericum perforatum are important secondary metabolites which have been widely utilized in medicine for a range of purposes. The use of methyl jasmonate (MeJA) elicitation for the enhancement of flavonoid production in cell suspension culture of H. perforatum would be an efficient alternative method for the flavonoid production.ResultsMeJA influenced the cells growth and flavonoid production. The optimal elicitation strategy was treatment of the cell cultures with 100 μmol/L MeJA on day 15, which resulted in the highest flavonoid production (280 mg/L) and 2.7 times of control cultures. The activities of catalase (CAT) were inhibited after MeJA treatment in the cell cultures, while the activities of phenylalanine ammonia lyase (PAL) increased, which led to the enhancement of flavonoid production.ConclusionMeJA elicitation is a useful method for the enhancement of flavonoid production in cell suspension culture of H. perforatum.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/3d958a4960fe84e2a1b3b5d63cceb5d681cd6af0

Citations in the corpus

• Mitigation of sodium chloride toxicity in Solanum lycopersicum L. by supplementation of jasmonic acid and nitric oxide (2018, unknown): “The increased synthesis of flavonoids due to JA supplementation supports the findings by Horbowicz et al. (2011), Ishihara et al. (2002), and Wang et al. (2015).”