Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

By S. Gelvin. Microbiology and Molecular Biology Reviews, 2003

1,382 citations recorded by the source corpus.

Subfields: Biotechnology, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/3d7adbce5f68289c3b3fd371f89cbc81340d6d28

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biotechnology

1. D. Becker
2. G. Chung
3. R. Harding
4. Vinay Kumar
5. A. Tyagi
6. Mary B. Taylor
7. C. Taylor
8. H. Daniell

In Molecular Biology

1. Jian-Feng Li
2. D. Becker
3. S. Delauré
4. G. Chung
5. R. Harding
6. Vinay Kumar
7. Ralph Bock
8. Katleen M. J. Butaye

In Plant Science

1. Jian-Feng Li
2. D. Becker
3. S. Delauré
4. G. Chung
5. R. Harding
6. Vinay Kumar
7. Katleen M. J. Butaye
8. A. Tyagi

Citations in the corpus

• Genetic modification: The production of recombinant pharmaceutical proteins in plants (2003, background): “Preliminary studies have indicated that the co-transformation of plants with a primary transgene and a viral-silencing suppressor notably increases transgene expression leve”
• Modern Biotechnology as an Integral Supplement to Conventional Plant Breeding: The Prospects and Challenges (2006, methodology): “The world’s major crops are being transformed by direct DNA delivery by microprojectile bombardment and other methods of direct gene transfer (e.g., Jauhar and Chibbar, 1999; Dahleen et al., 2001; Muthukrishnan et al., 2001; Patnaik and Khurana, 2001; Repellin et al., 2001; Gelvin, 2003; Sharma et a”
• Phytohormones and their metabolic engineering for abiotic stress tolerance in crop plants (2016, positive): “In Agrobacterium , bacterial genes can be successfully replaced with gene(s) of interest, and interestingly, this replacement does not affect the transformation process or frequency [133,134].”
• Particle bombardment and the genetic enhancement of crops: myths and realities (2005, background): “Several investigators have recently suggested that particle bombardment will inevitably be supplanted by transformation methods using the soil bacterium Agrobacterium tumefaciens and its relatives, at least for the production of commercial genetically enhanced crops (Gelvin 2003; Tzfira and Citovsky”
• Approaches to Minimize Variation of Transgene Expression in Plants (2005, background): “biolistics and electroporation) contain a large number of transgene copies (reportedly even up to 100 copies) whereas Agrobacteriummediated transformation leads to the insertion of fewer transgene copies (<10) and a higher occurrence of single-copy integrants (Hansen and Wright 1999; Koprek et al. 2”
• Perspectives for nano-biotechnology enabled protection and nutrition of plants. (2011, neutral): “Delivery of geneticmaterial such as DNA and small interfering RNA is important for the development of pest, pathogen and stress resistant strains of crop plants by alteration of gene expression (Gelvin, 2003; Price and Gatehouse, 2008).”
• Review of empirical and emerging breeding methods and tools for yam ( Dioscorea spp.) improvement: Status and prospects (2019, background): “…ability to transfer low copies of DNA fragments carrying the desirable genes at higher efficiencies with minimal cost as well as the transfer of very large DNA fragments with low rearrangement, Agrobacterium-mediated gene delivery system is the most preferred (Gelvin, 2003; Shibata & Liu, 2000).”
• The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species (2009, background): “Agrobacterium-mediated transient assays harness the natural capability of Agrobacterium to transfer foreign DNA into plant cells with an intact cell wall [8].”
• Factors affecting somatic embryogenesis and transformation in modern plant breeding (2010, methodology): “The binary vector can be cloned in E. coli and transformed into an Agrobacterium strain containing a helper plasmid (Gelvin, 2003).”
• ALGAL TRANSGENICS IN THE GENOMIC ERA 1 (2005, background): “Agrobacterium-mediated transformation has been shown to be applicable to a wide range of plant and fungal species (Gelvin 2003) and has now been used to transform Porphyra (Cheney 2001), the giant unicellular alga Acetabularia acetabulum (D. Mandoli personal communication) and C. reinhardtii (Kumar…”
• Use of non-integrating Zm-Wus2 vectors to enhance maize transformation (2020, background): “Agrobacterium-mediated T-DNA transformation involves transient T-DNA gene expression within 36–48 h post infection, followed by stable T-DNA integration into the plant genome (Yoshioka et al. 1996; Gelvin 2003).”