Gene expression and activity of antioxidant enzymes in barley (Hordeum vulgare L.) under controlled severe drought

By A. Harb, D. Awad, N. Samarah. 2015

106 citations recorded by the source corpus.

The objective of the present study was to determine the activity of antioxidant enzymes: superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) and the expression of their genes in two barley genotypes under controlled severe drought. To fulfill this objective, 21-day-old barley plants of two genotypes: Rum and Yarmouk were exposed to controlled severe drought (25% field capacity) for 2, 9, and 16 days. The activity of SOD was significantly high in Rum genotype after 2 days of drought treatment. In Yarmouk genotype, the activity of APX was significantly high after 2 and 9 days of drought treatment. In Rum genotype, CAT2 was upregulated after 9 days of drought treatment and SOD and APX were upregulated after 16 days of drought treatment, whereas CAT2, SOD, and APX were upregulated in Yarmouk genotype after 2 days of drought treatment. The results indicate a unique pattern of activity and gene expression of the antioxidant enzymes in the two barley genotypes under controlled severe drought. Moreover, the data suggest that each genotype utilizes different molecular and biochemical responses under the same drought conditions.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/3c715f94448c278e7ebb2e5f50ec6918e2204451

Citations in the corpus

• Molecular responses to drought stress in plants (2017, unknown): “In order to cope with continuous ROS production under stresses, plants have developed battery of enzymatic and non-enzymatic antioxidants that function as an extremely efficient cooperative system (Harba et al. 2015).”