Methodology for quantitative determination of the carbohydrate composition of brown seaweeds (Laminariaceae)
By D. Manns, A. L. Deutschle, B. Saake, Anne S. Meyer. 2014
129 citations recorded by the source corpus.
The monosaccharide composition of four different samples of brown seaweeds Laminaria digitata and Saccharina latissima were compared by different high performance anion exchange chromatography (HPAEC) methods after different acid hydrolysis treatments or a cellulase treatment. A two-step treatment of 72% (w/w) H2SO4 + 4% (w/w) H2SO4 performed best, but cellulase treatment released more glucose than acid treatments. HPAEC with pulsed amperometric detection (PAD) allowed quantification of all present neutral sugars and the sugar alcohol mannitol. Furthermore, the use of guluronic, glucuronic, and galacturonic acid as standards enabled quantification of the uronic acids. A complete map of amino acids, fatty compounds, minerals, and ash was also achieved. L. digitata and S. latissima harvested in Denmark April (Baltic Sea, 2012) were dominated by alginic acid and ash (each ∼30% by weight (w/w) of the dry matter) and 10% (w/w) protein. In contrast, the dominant compound of L. digitata harvested in August (North Sea, 2012) was glucose constituting 51% w/w of the dry matter, and with 16% w/w alginic acid. Washing prior to analysis mainly removed salts.
Subfields: Aquatic Science, Food Science, Nutrition and Dietetics
View Paper: https://www.semanticscholar.org/paper/3be3c3f60e9fe79df95e619e2ec761eea4fb994a
How researchers see this paper
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- M. M. Nielsen
- D. Manns
- Annette Bruhn
- E. Kostas
- Anne S. Meyer
- B. Saake
- J. Adams
- H. Ruiz
Citations in the corpus
- Compositional variations of brown seaweeds Laminaria digitata and Saccharina latissima in Danish waters (2017, methodology): “digitata harvested from the Danish North Sea in August 2012 contained 51% by weight of dry matter (w/w) glucans (Manns et al. 2014).”
- Seaweed Bioethanol Production: A Process Selection Review on Hydrolysis and Fermentation (2018, unknown): “Author Contributions: Contributions from the authors include: conceptualization, F.O.; writing—original draft preparation, F.O. and A.T.; writing—review and editing, M.M., F.K., A.T. and F.O. Funding: This research was funded by DANIDA Fellowship Centre (Denmark’s development cooperation) grant numb”
- Macroalgal biorefinery concepts for the circular bioeconomy: A review on biotechnological developments and future perspectives (2021, background): “The biochemical composition of brown species of macroalgae is highly complex, and possess a unique yet heterogeneous carbohydrate composition present in high concentrations (34–76 % dw) [63].”