Short-term dynamics in microphytobenthos distribution and associated extracellular carbohydrates in surface sediments of an intertidal mudflat

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The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides (2004, background): “…years, there have been a number of studies that have adopted a warm water treatment to extract putative extracellular polysaccharides from marine benthic diatoms (Staats et al., 1999, 2000a,b; de Brouwer & Stal, 2001, 2002; de Brouwer et al., 2002; Wolfstein & Stal, 2002; Wolfstein et al., 2002).”
ENVIRONMENTAL EFFECTS ON EXOPOLYMER PRODUCTION BY MARINE BENTHIC DIATOMS: DYNAMICS, CHANGES IN COMPOSITION, AND PATHWAYS OF PRODUCTION 1 (2004, methodology): “The extracellular carbohydrate exudates produced by benthic diatoms can be methodologically separated into different fractions using precipitation in ethanol or by ultrafiltration (Smith and Underwood 2000, de Brouwer and Stal 2001).”
DAILY FLUCTUATIONS OF EXOPOLYMERS IN CULTURES OF THE BENTHIC DIATOMS CYLINDROTHECA CLOSTERIUM AND NITZSCHIA SP. (BACILLARIOPHYCEAE)1 (2002, methodology): “It has frequently been observed that EPS production by natural communities of diatoms is light dependent (de Winder et al 1999, Taylor et al. 1999, Staats et al. 2000a, de Brouwer and Stal 2001).”