Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis.

By Youfa Cheng, Xinhua Dai, Yunde Zhao. Genes & Development, 2006

1,154 citations recorded by the source corpus.

Auxin biosynthesis in plants has remained obscure although auxin has been known for decades as a key regulator for plant growth and development. Here we define the YUC gene family and show unequivocally that four of the 11 predicted YUC flavin monooxygenases (YUC1, YUC2, YUC4, and YUC6) play essential roles in auxin biosynthesis and plant development. The YUC genes are mainly expressed in meristems, young primordia, vascular tissues, and reproductive organs. Overexpression of each YUC gene leads to auxin overproduction, whereas disruption of a single YUC gene causes no obvious developmental defects. However, yuc1yuc4, yuc2yuc6, all of the triple and quadruple mutants of the four YUC genes, display severe defects in floral patterning, vascular formation, and other developmental processes. Furthermore, inactivation of the YUC genes leads to dramatically reduced expression of the auxin reporter DR5-GUS in tissues where the YUC genes are expressed. Moreover, the developmental defects of yuc1yuc4 and yuc1yuc2yuc6 are rescued by tissue-specific expression of the bacterial auxin biosynthesis gene iaaM, but not by exogenous auxin, demonstrating that spatially and temporally regulated auxin biosynthesis by the YUC genes is essential for the formation of floral organs and vascular tissues.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/389c0acdcba5ad622dac4c05b2fe621cfad03fb6

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Citations in the corpus

The main auxin biosynthesis pathway in Arabidopsis (2011, background): “yuc1 yuc2 yuc6 and yuc1 yuc2 yuc4 yuc6 were generated from yuc1/− yuc2/+ yuc4/+ yuc6/− plants, wei8-1 tar2-1 from wei8-1/− tar2-1/+, and wei8-1 tar2-2 from wei8-1/− tar2-2/+ (19, 26).”
Auxin biosynthesis and its role in plant development (2010, background): “De novo auxin production is highly localized and local auxin biosynthesis plays a key role in shaping local auxin gradients (8, 9, 56, 60).”
Auxin Regulates Arabidopsis Anther Dehiscence, Pollen Maturation, and Filament Elongation (2008, background): “Stamen development in these mutants could be rescued by expression ofthebacterial auxinbiosynthesisgene iaaMunderthecontrolof YUC6 promoter ( Cheng et al., 2006 ).”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “Although these pathways are not yet fully understood, several biosynthetic genes have been identified, such as the YUCCA gene family (Steven, 2001; Zhao et al., 2001; Cheng et al., 2006).”
Auxin control of root development. (2010, background): “Despite being the first plant hormone identified, experimental evidence detailing multiple auxin biosynthetic pathways has only recently been reported (Zhao et al. 2001; Zhao et al. 2002; Woodward and Bartel 2005; Cheng et al. 2006; Seidel et al. 2006; Normanly 2009).”
Rapid synthesis of auxin via a new tryptophan-dependent pathway is required for shade avoidance in plants. (2008, neutral): “Of interest, the expression pattern of SAV3 in the shoot was similar to that of DR5::GUS, an artificial auxin reporter gene construct, whose expression is thought to reflect the levels of free auxin (Aloni et al., 2003; Cheng et al., 2006; Mallory et al., 2005; Sabatini et al., 1999).”
Auxin Control in the Formation of Adventitious Roots (2011, positive): “YUCCA1 gene encodes a flavin monooxygenase suitable for converting tryptamine in N-hydroxyl tryptamine in vitro (Zhao et al. , 2001, 2002) and belongs to a family of YUCflavin mono-oxigenases from which 4 have a role in auxin biosynthesis (Cheng et al. , 2006).”
Auxin Synthesized by the YUCCA Flavin Monooxygenases Is Essential for Embryogenesis and Leaf Formation in Arabidopsis[W] (2007, methodology): “We performed GUS staining as previously described (Cheng et al., 2006).”
TAA1-mediated auxin biosynthesis is essential for hormone crosstalk and plant development. (2008, background): “The expression of YUC genes in gynoeciums and the valveless phenotypes of double and triple yuc mutants have suggested a role for auxin production in the formation of this auxin gradient (Cheng et al., 2006).”
A Role for Auxin in Flower Development (2007, background): “Recent studies on roles of the YUCCA (YUC) family of flavin monooxygenases in auxin biosynthesis provide unambiguous evidence that auxin is necessary for the formation of flowers (Zhao et al. 2001; Tobena-Santamaria et al. 2002; Cheng et al. 2006).”
Conversion of tryptophan to indole-3-acetic acid by TRYPTOPHAN AMINOTRANSFERASES OF ARABIDOPSIS and YUCCAs in Arabidopsis (2011, methodology): “Our previous work on yuc mutants mainly focused on the development of aerial parts of Arabidopsis (6, 10).”
Auxin: the looping star in plant development. (2008, background): “The exact sites of auxin biosynthesis are unknown, but the identification of molecular components of auxin biosynthesis revealed the existence of at least two separate biosynthesis pathways (6, 23, 145).”