Landscape and Dynamics of Single Immune Cells in Hepatocellular Carcinoma.
By Qiming Zhang, Yao He, Nan Luo, Shashank J. Patel, Yanjie Han, Ranran Gao, Madhura Modak, Sebastian Carotta, C. Haslinger, D. Kind and 15 more. Cell, 2019
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The immune microenvironment of hepatocellular carcinoma (HCC) is poorly characterized. Combining two single-cell RNA sequencing technologies, we produced transcriptomes of CD45+ immune cells for HCC patients from five immune-relevant sites: tumor, adjacent liver, hepatic lymph node (LN), blood, and ascites. A cluster of LAMP3+ dendritic cells (DCs) appeared to be the mature form of conventional DCs and possessed the potential to migrate from tumors to LNs. LAMP3+ DCs also expressed diverse immune-relevant ligands and exhibited potential to regulate multiple subtypes of lymphocytes. Of the macrophages in tumors that exhibited distinct transcriptional states, tumor-associated macrophages (TAMs) were associated with poor prognosis, and we established the inflammatory role of SLC40A1 and GPNMB in these cells. Further, myeloid and lymphoid cells in ascites were predominantly linked to tumor and blood origins, respectively. The dynamic properties of diverse CD45+ cell types revealed by this study add new dimensions to the immune landscape of HCC.
Subfields: Immunology, Oncology
View Paper: https://www.semanticscholar.org/paper/38364567e8366cbac56595bc8a9ec80a7ae469d4
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Citations in the corpus
- Deciphering cell–cell interactions and communication from gene expression (2020, methodology): “Although this method can be applied to bulk data, microdissected or single-cell data sets are preferred as they capture the full heterogeneity of expression across cells 19,48,49,55,60,62 , resulting in clearer differences in expression 92 .”