Direct Interaction between Mammalian DNA Polymerase β and Proliferating Cell Nuclear Antigen*

By P. Kedar, Soon-jong Kim, A. Robertson, E. Hou, R. Prasad, J. Horton, Samuel H. Wilson. Journal of Biological Chemistry, 2002

119 citations recorded by the source corpus.

Proliferating cell nuclear antigen (PCNA) plays an essential role in nucleic acid metabolism as a component of the DNA replication and DNA repair machinery. As such, PCNA interacts with many proteins that have a sequence motif termed the PCNAinteracting motif (PIM) and also with proteins lacking a PIM. Three regions in human and rat DNA polymerases β (β-pol) that resemble the consensus PIM were identified, and we show here that β-polymerase and PCNA can form a complex both in vitro and in vivo. Immunoprecipitation experiments, yeast two-hybrid analysis, and overlay binding assays were used to examine the interaction between the two proteins. Competition experiments with synthetic PIM-containing peptides suggested the importance of a PIM in the interaction, and studies of a β-polymerase PIM mutant, H222A/F223A, demonstrated that this alteration blocked the interaction with PCNA. The results indicate that at least one of the PIM-like sequences in β-polymerase appears to be a functional PIM and was required in the interaction between β-polymerase and PCNA.

Subfields: Materials Chemistry, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/34c814d61c62708e07eae532c754d789b51bb7b7

Citations in the corpus

• Proliferating cell nuclear antigen (PCNA): a key factor in DNA replication and cell cycle regulation. (2011, unknown): “Additional, new evidence supporting the role of PCNA in short-patch BER was provided by experiments showing recruitment of XRCC1 protein by PCNA to replication foci and interaction of PCNA with DNA pol b (Kedar et al., 2002; Fan et al., 2004).”