Expression of intermediate filament nestin in blood vessels of neural and non-neural tissues.

By J. Mokry, J. Ehrmann, J. Karbanová, D. Čížková, T. Soukup, J. Suchánek, S. Filip, Z. Kolár̂. 2008

59 citations recorded by the source corpus.

Our previous findings performed in rat tissues demonstrated that intermediate filament nestin is expressed in endothelial cells of newly formed blood vessels of developing organs and neural transplants. The aim of the present study was to identify other cellular markers expressed in nestin-positive (nestin+) blood vessels. To reach this goal we performed double immunofluorescent study to co-localize nestin with endothelium-specific markers (CD31, CD34 II, vimentin) or markers of perivascular cells (GFAP, SMA) in paraffin-embedded sections of normal human brain tissue, low- and high-grade gliomas, postinfarcted heart and samples of non-neural tumours. Our findings documented that all the samples examined contained blood vessels with different ratio of nestin+ endothelial cells. Double immunostaining provided unambiguous evidence that endothelial cells expressed nestin and allowed them to distinguish from other nestin+ elements (perivascular astrocytic endfeet, undifferentiated tumour cells, smooth muscle cells and pericytes). Nestin+ endothelium was not confined only to newly formed capillaries but was also observed in blood vessels of larger calibres, frequently in arterioles and venules. We conclude that nestin represents a reliable vascular marker that is expressed in endothelial cells. Elevation of nestin expression likely corresponds to reorganization of intermediate filament network in the cytoskeleton of endothelial cells in the course of their maturation or adaptation to changes in growing tissues.

Subfields: Developmental Neuroscience, Genetics, Molecular Biology

Citations in the corpus

• Human Hippocampal Neurogenesis Persists throughout Aging (2018, neutral): “, 2014) showed distinct perikaryon and multipolar processes, contacting nestin+ remodeling capillaries (Mokrý et al., 2008; Salehi et al., 2008) in the neurogenic niche of the subgranular zone (SGZ, Figures 1E–1H and Figures 4A–4C), as we reported earlier (Boldrini et al.”
• Benzodiazepines And The Potential Trophic Effect Of Antidepressants On Dentate Gyrus Cells In Mood Disorders (2014, unknown): “Nestin is also a marker of neovascularization, and it labels capillaries and newly formed vessels after ischemia until the stroke is remodeling, while it is not expressed in absence of vascular response (Mokry et al., 2008; Salehi et al., 2008).”