Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency.

By R. Kapust, J. Tözsér, J. Fox, D. Anderson, S. Cherry, T. Copeland, D. Waugh. Protein Engineering, 2001

881 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/3275a2394f5b66e4a06f51166a63a3c261bd5488

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Citations in the corpus

G3BP1 is a tunable switch that triggers phase separation to assemble stress granules (2020, neutral): “The eluted proteins were incubated with TEV protease at 4°C overnight (Kapust et al., 2001).”
Intracellular Detection and Evolution of Site-Specific Proteases Using a Genetic Selection System (2012, methodology): “The TEV-Pr gene was amplified from the pRK793 vector [38] expressing autoinactivationresistant mutant S219V.”
An Overview of Enzymatic Reagents for the Removal of Affinity Tags (2011, background): “The 27 kDa catalytic domain of TEV protease readily cleaves itself near its C-terminus to generate a truncated enzyme with greatly diminished activity [63,18].”
Enhancing the stability and solubility of TEV protease using in silico design (2007, methodology): “At present, recombinant TEV is generally soluble at concentrations below ;1 mg/mL and is typically stored with up to 50% (v/v) glycerol (Kapust et al. 2001).”
Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries (2013, methodology): “Because the original wild-type protease undergoes strong autolysis, a well-characterized variant containing a S219P mutation to significantly increase its stability was used in our research, which is annotated as Tobacco Etch Virus protease (TEV-P) (26).”
Diverse RNA-Binding Proteins Interact with Functionally Related Sets of RNAs, Suggesting an Extensive Regulatory System (2008, methodology): “David Waugh kindly provided TEV protease expression plasmid.”