Quantitative neuroanatomy for connectomics in Drosophila
By C. Schneider-Mizell, S. Gerhard, Mark Longair, Tom Kazimiers, Feng Li, Maarten F. Zwart, A. Champion, F. Midgley, R. Fetter, Stephan Saalfeld and 1 more. bioRxiv, 2015
304 citations recorded by the source corpus.
Neuronal circuit mapping using electron microscopy demands laborious proofreading or reconciliation of multiple independent reconstructions. Here, we describe new methods to apply quantitative arbor and network context to iteratively proofread and reconstruct circuits and create anatomically-enriched wiring diagrams. We measured the morphological underpinnings of connectivity in new and existing reconstructions of Drosophila sensorimotor (larva) and visual (adult) systems. Synaptic inputs were preferentially located on numerous small, microtubule-free “twigs” which branch off a single microtubule-containing “backbone”. Omission of individual twigs accounted for 96% of errors. However, the synapses of highly connected neurons were distributed across multiple twigs. Thus the robustness of a strong connection to detailed twig anatomy was associated with robustness to reconstruction error. By comparing iterative reconstruction to the consensus of multiple reconstructions, we show that our method overcomes the need for redundant effort through the discovery and application of relationships between cellular neuroanatomy and synaptic connectivity.
Subfields: Cellular and Molecular Neuroscience, Cognitive Neuroscience
View Paper: https://www.semanticscholar.org/paper/31b64b29c1bb86ae34ebbbdb7816acc37fac853a
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In Cellular and Molecular Neuroscience
Citations in the corpus
- Statistical Inference on Random Dot Product Graphs: a Survey (2017, methodology): “Our MB connectome was obtained via serial section transmission electron microscopy of an entire larval Drosophila nervous system [75, 84].”
- A Complete Electron Microscopy Volume of the Brain of Adult Drosophila melanogaster (2017, methodology): “Therefore we also reconstructed the contralateral MB-CP2 neuron and its synapses to assess the symmetry of its morphology and connectivity, a previously used technique to validate the reconstruction accuracy of known and previously unidentified cell types in the Drosophila larval EM volume (Eichler ”