Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

By M. Reetz, J. D. Carballeira. Nature Protocols, 2007

788 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/318de3e9e3b14e890adc7cafbb6187e1c38b87c9

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomedical Engineering

1. John M. Woodley
2. R. Sheldon

In Molecular Biology

1. Hak-Sung Kim
2. J. Choi
3. Sang-Soo Han
4. Tingjian Chen
5. F. Romesberg
6. John M. Woodley
7. R. Sheldon

In Spectroscopy

1. Hak-Sung Kim
2. J. Choi
3. Sang-Soo Han

Citations in the corpus

• Directed Polymerase Evolution (2013, background): “In ISM, a limited number of residues within focused regions of the protein of interest are randomized in separated libraries and then subjected to selection or screening [47].”
• Industrial applications of enzyme biocatalysis: Current status and future aspects. (2015, background): “B-FIT targets amino acids with high temperature factor (B-factor) in crystal structure, indicating high thermal flexibility (Reetz et al., 2006a; Reetz and Carballeira, 2007).”