Three-Dimensional Culture Systems in Cancer Research: Focus on Tumor Spheroid Model
By S. Nath, G. Devi. Pharmacology and Therapeutics, 2016
821 citations recorded by the source corpus.
Cancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically relevant cell-cell and cell-matrix interactions, gene expression and signaling pathway profiles, heterogeneity and structural complexity that reflect in vivo tumors. In recent years, development of various 3D models have improved the study of host-tumor interaction and use of high-throughput screening platforms for anti-cancer drug discovery and development. This review attempts to summarize the various 3D culture systems, with an emphasis on the most well characterized and widely applied model - multicellular tumor spheroids. This review also highlights the various techniques to generate tumor spheroids, methods to characterize them, and its applicability in cancer research.
Subfields: Biomedical Engineering, Cell Biology, Oncology
View Paper: https://www.semanticscholar.org/paper/310b9894cde283e7bf5bf576833ca7861bca65f4
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Citations in the corpus
- Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning (2018, background): “to perform drug screens and facilitate drug development in a patient-centered manner (Stadler et al., 2015; Nath and Devi, 2016; Pauli et al., 2017; Weeber et al., 2017).”
- Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture (2018, methodology): “investigated conventional methods, novel non-microfluidic methods and on-chip microfluidic methods for spheroid formation, while also highlighting applications of tumor spheroids in cancer research [24].”
- 3D tumor spheroids as in vitro models to mimic in vivo human solid tumors resistance to therapeutic drugs (2018, background): “These natural biomaterials are attractive for in vitro applications because they are biocompatible, contain cell adhesion sites, as well as endogenous chemokines and growth factors, which contribute to enhancing the cells viability and growth (Nath & Devi, 2016).”