DNA Barcoding for Honey Biodiversity

By A. Valentini, C. Miquel, P. Taberlet. 2010

112 citations recorded by the source corpus.

Honey is produced by honeybees from nectar and from secretions of living plants. It reflects the honeybees’ diet and the local plant communities. Honey also shows different plant compositions in different geographical locations. We propose a new method for studying the plant diversity and the geographical origin of honey using a DNA barcoding approach that combines universal primers and massive parallel pyrosequencing. To test this method we use two commercial honeys, one from a regional origin and one composed of a worldwide mix of different honeys. We demonstrate that the method proposed here is fast, simple to implement, more robust than classical methods, and therefore suitable for analyzing plant diversity in honey.

Subfields: Ecology, Evolution, Behavior and Systematics, Insect Science

View Paper: https://www.semanticscholar.org/paper/2fd406b9ba1b7e77dbfc935e569350dc3f69500c

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

1. Krista M. Ruppert
2. Richard J. Kline
3. Md Saydur Rahman

Citations in the corpus

• Past, present, and future perspectives of environmental DNA (eDNA) metabarcoding: A systematic review in methods, monitoring, and applications of global eDNA (2019, background): “Additionally, the nature of honey prevents DNA degradation, so multiple, longer primer sets could be used if certain regions are indistinguishable with the universal primers (Valentini et al., 2010).”