Comparison of ITS1 and ITS2 rDNA in 454 sequencing of hyperdiverse fungal communities

By A. Bazzicalupo, Miklós Bálint, Imke Schmitt. 2013

149 citations recorded by the source corpus.

Subfields: Cell Biology, Ecology, Evolution, Behavior and Systematics, Plant Science

View Paper: https://www.semanticscholar.org/paper/2fc7f28b429dd2f95d324b0d845de0e579cec43e

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cell Biology

1. Ian A. DICKIE
2. R. Holdaway

In Ecology, Evolution, Behavior and Systematics

1. Imke Schmitt
2. Ian A. DICKIE
3. H. Buckley
4. R. Smissen
5. R. Cruickshank
6. T. Buckley
7. R. Holdaway
8. D. Orlovich

In Plant Science

1. Miklós Bálint
2. Ian A. DICKIE
3. R. Smissen
4. R. Holdaway

Citations in the corpus

• Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples (2018, methodology): “…(ITS) region 1 (ITS1, located between 18S and 5.8S rRNA genes) was the more commonly targeted (e.g. Bellemain et al. 2010; Singh et al. 2012; Bazzicalupo et al. 2013; Song et al. 2015), with more recent studies switching to the ITS2 region (between 5.8S and 28S rRNA genes; Bazzicalupo et…”
• Illumina metabarcoding of a soil fungal community (2013, background): “Even if the full ITS is amplified, the most common 454 chemistry recovers either ITS1 or ITS2 in full length, while the sequenced fraction of the other ITS subfragment is too short for inferences (Bálint et al., 2013; Bazzicalupo et al., 2013).”