Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions

By Norma J. Greenfield. Nature Protocols, 2006

877 citations recorded by the source corpus.

Subfields: Materials Chemistry, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/2f9135362ff8540769328a7af24c8b0e051becfa

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Materials Chemistry

1. S. Keller
2. G. Kemmer

In Molecular Biology

1. S. Keller
2. G. Kemmer
3. Norma J. Greenfield

Citations in the corpus

• Using circular dichroism spectra to estimate protein secondary structure (2007, background): “It is very useful to determine how many different states contribute to the changes in CD as a function of ligand or temperatur”
• Nonlinear least-squares data fitting in Excel spreadsheets (2010, methodology): “In this experiment, the small globular protein RNAse A was unfolded by raising the temperature from 24 to 94 °C, and loss of secondary structure was monitored by recording the circular dichroism (CD) signal in the far-UV range (see ref”