Cell sensitivity assays: the MTT assay.

By J. van Meerloo, G. Kaspers, J. Cloos. Methods in molecular biology, 2011

2,222 citations recorded by the source corpus.

The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay is based on the conversion of MTT into formazan crystals by living cells, which determines mitochondrial activity. Since for most cell populations the total mitochondrial activity is related to the number of viable cells, this assay is broadly used to measure the in vitro cytotoxic effects of drugs on cell lines or primary patient cells. In this chapter the protocol of the assay is described including important considerations relevant for each step of the assay as well as its limitations and possible applications.

Subfields: Genetics, Molecular Biology, Organic Chemistry

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Citations in the corpus

• MTT assay for cell viability: Intracellular localization of the formazan product is in lipid droplets. (2012, methodology): “Van Meerloo J, Kaspers GJ, Cloos J. Cell sensitivity assays: the MTT assay.”