Molecular evolution by staggered extension process (StEP) in vitro recombination

By Huimin Zhao, L. Giver, Z. Shao, J. Affholter, Frances H. Arnold. 1998

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Citations in the corpus

Evolutionary context-integrated deep sequence modeling for protein engineering (2020, background): “Directed evolution is a protein engineering technique that circumvents this intractability in a greedy way [12–14] without the availability of protein structure.”
The protein folding problem. (2008, background): “Novel proteins are now designed as variants of existing proteins (43,94,99,145,173,243), or from broadened alphabets of nonnatural amino acids (226), or de novo (129) (Figure 2).”
Recent advances in the bioremediation of persistent organic pollutants via biomolecular engineering (2005, background): “In essence, directed evolution involves the creation of a diverse library of gene variants through random mutagenesis, such as error prone PCR or gene recombination techniques, such as the in vitro staggered extension process (StEP) recombination [8] and in vivo DNA shuffling [9], followed by select”
Thermophilic Adaptation of Proteins (2001, background): “Recent technical advances such as DNA shuffling (Stemmer, 1994a,b) or the staggered extension process (Zhao et al., 1998) allow the recombination of beneficial mutations that are created in the first rounds of directed evolution.”
Antibiotic resistance plasmids in wastewater treatment plants and their possible dissemination into the environment (2010, methodology): “The staggered extension process recombination is performed through series of polymerase-catalyzed primer extensions, generating cross hybridization during growing of gene fragments (Zhao et al., 1998).”
Machine learning in protein engineering (2018, background): “Recombination methods can make larger jumps in sequence space while preserving a large fraction of functional sequences by only considering diversity from within a set of related proteins (Zhao et al., 1998).”