High efficiency degradation of alkanes and crude oil by a salt-tolerant bacterium Dietzia species CN-3

By Weiwei Chen, Junde Li, Xiang-Sheng Sun, J. Min, Xiaoke Hu. 2017

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Abstract A bacterial strain, CN-3, was isolated from petroleum-contaminated sediment in Bohai Bay, China. Morphological, physiological and phylogenetic analyses indicated that the strain belonged to the genus Dietzia . It was capable of utilizing a wide range of alkanes (C 14 –C 31 ), aromatic compounds and crude oil as the sole carbon and energy sources. The bacterium showed high degradation rate (>80%) of the saturated hydrocarbons of crude oil in the pH range of 6–9. The degradation rate was hardly affected at a NaCl concentration up to 85 g L −1 , indicating that the strain can tolerate high salinities. The real-time quantitative Polymerase Chain Reaction (PCR) results showed that the CYP153 genes could be significantly induced by C 14 , C 15 , C 16 , C 26 and pristane, whereas the transcription level of alk B gene was increased moderately with C 14 , C 15 , C 16 , C 26 and pristane induction condition. A high degradation extent, wide substrate range and facile conditions enhanced the potential for this bacterial strain to be used in the bioremediation of crude oil pollution.

Subfields: Molecular Biology, Pollution, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/2e8fc9e848175f9daf68d45e2e77053b3ab34583