Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy

By Raju Tomer, K. Khairy, F. Amat, Philipp J. Keller. Nature Methods, 2012

526 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Biophysics, Structural Biology

View Paper: https://www.semanticscholar.org/paper/2cdb970c42ad1e88e10da3a572419ce55d3f065c

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In Biomedical Engineering

1. John M. Girkin
2. Mariana T Carvalho

In Biophysics

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In Structural Biology

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Citations in the corpus

• Advanced methods of microscope control using μManager software. (2014, background): “High speed sCMOS cameras have proven useful for imaging three-dimensional evolving specimens [5] and advanced applications such as super-resolution microscopy [6].”
• Simple, scalable proteomic imaging for high-dimensional profiling of intact systems (2015, unknown): “We imaged this larger volume using a custom-built, highspeed light-sheet microscope (Tomer et al., 2012; Tomer et al., 2014) within 2 hours and observed uniform labeling of all myelinated fibers across the entire tissue (Figure 5J; Movie S4).”
• Forces in tissue morphogenesis and patterning. (2013, unknown): “In recent years, considerable progress has been made in the development of image acquisition tools to record dynamic changes in cell and tissue shapes at high spatial and temporal resolution and in the development of image analysis tools to quantify cell/tissue dynamics (Montero et al., 2005; Kwon e”
• The light-sheet microscopy revolution (2018, methodology): “In the multidirectional illumination route, there are the mSPIM, MuVi-SPIM [36] and SIMViewSPIM [73], combining multiple illumination directions and multiple view directions to create a Multiview [35] image of”
• Lattice Light Sheet Microscopy: Imaging Molecules to Embryos at High Spatiotemporal Resolution (2014, unknown): “This approach has proven powerful for noninvasive four-dimensional (4D) imaging of embryos at single-cell resolution (5, 6); however, conventional light sheets created with Gaussian beams (Fig.”
• Building quantitative, three dimensional atlases of gene expression and morphology at cellular resolution (2013, background): “50 Depending on the orientation of the objective lenses, the optical penetration through the sample can be doubled (objectives at 180 ◦ orientation), or the axial resolving power of one lens can be increased by the second lens (objectives at 90 ◦ orientation).”