Imipenem-EDTA Disk Method for Differentiation of Metallo-β-Lactamase-Producing Clinical Isolates of Pseudomonas spp. and Acinetobacter spp

By D. Yong, Kyungwon Lee, J. Yum, H. Shin, G. Rossolini, Y. Chong. Journal of Clinical Microbiology, 2002

662 citations recorded by the source corpus.

ABSTRACT Rapid detection of metallo-β-lactamase (MBL)-producing gram-negative bacilli is necessary to prevent their dissemination. The method using a disk with imipenem plus 750 μg of EDTA differentiated all MBL-producing pseudomonads, and the sensitivity and specificity for acinetobacters were 95.7 and 91.0%, respectively. The imipenem-EDTA disks were stable for 12 and 16 weeks at 4 and −20°C, respectively.

Subfields: Molecular Biology, Molecular Medicine, Pollution

View Paper: https://www.semanticscholar.org/paper/2cb8e0fcfebaa9c11d4bffb47abed9f18ffe6050

Citations in the corpus

• Carbapenemases: the Versatile β-Lactamases (2007, neutral): “The disk approximation test with EDTA or 2-mercaptoproionic acid is often used as a screen for metallo- -lactamase producers (8, 248).”