Highly efficient gene delivery by mRNA electroporation in human hematopoietic cells: superiority to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for tumor antigen…

By V. V. Tendeloo, P. Ponsaerts, F. Lardon, G. Nijs, M. Lenjou, C. Broeckhoven, D. R. Bockstaele, Z. Berneman. 2001

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Simultaneous Activation of Viral Antigen-specific Memory CD4+ and CD8+ T-cells Using mRNA-electroporated CD40-activated Autologous B-cells (2006, methodology): “Electroporation of mRNA was carried out as described previously with minor modifications.(21) Briefly, before electroporation, 10 10(6) CD40-B cells were washed twice with Optimix washing solution (EquiBio, Ashford, Middlesex, UK) and resuspended in Optimix electroporation buffer (EquiBio).”
Multiplex genome editing to generate universal CAR T cells resistant to PD1 inhibition (2016, methodology): “T cells were electroporated as described previously (15, 16).”
Highly efficient reprogramming to pluripotency and directed differentiation of human cells using synthetic modified mRNA (2010, background): “Indeed, the use of RNA transfection to express cancer or pathogen antigens for immunotherapy is already an active research area (Rabinovich et al., 2006, 2008; Van den Bosch et al., 2006; Weissman et al., 2000), and such approaches may benefit from the nonimmunogenic properties of modified RNAs.”