GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes.

By A. Walhout, G. Temple, M. Brasch, J. Hartley, Monique A Lorson, S. V. D. Heuvel, Marc Vidal. Methods in Enzymology, 2000

676 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/2b20c92fd55f5afcacc3701a36d5c77076ba0ee4

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In Molecular Biology

1. Marc Vidal
2. Hui Ge
3. Tetsuya Kimura
4. Drew Endy
5. S. Ishiguro
6. T. Nakagawa
7. D. Hunter
8. Jay D. Keasling

Citations in the corpus

• Identification of potential interaction networks using sequence-based searches for conserved protein-protein interactions or "interologs". (2001, methodology): “By comparison, an average of five interactors per bait typically is obtained using a worm AD-cDNA library representing ∼ 19,000 genes (2.6 (cid:1) 10 (cid:2) 4 ) (Walhout et al. 2000a; Davy et al. 2001).”
• The Tol2kit: A multisite gateway‐based construction kit for Tol2 transposon transgenesis constructs (2007, background): “…when combined with libraries of regulatory or coding sequences, including Caenorhabditis elegans open reading frame (ORF) and promoter collections (Walhout et al., 2000; Dupuy et al., 2004), and ORF collections for human (Lamesch et al., 2007), Schizosaccharomyces pombe (Matsuyama et al., 2006),…”
• Engineering BioBrick vectors from BioBrick parts (2008, methodology): “For example, several genomewide collections of open reading frames (ORFeomes) have been compiled using the Gateway® cloning system [32-34].”
• FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method (2011, methodology): “These methods include TA cloning [2], ligation independent cloning with T4 DNA polymerase [3,4], GATEWAY recombinational cloning [5], and more recent sequence- and ligation-independent cloning kits, such as CloneEZ (GenScript USA Inc.”
• Gateway Vectors for Plant Genetic Engineering: Overview of Plant Vectors, Application for Bimolecular Fluorescence Complementation (BiFC) and Multigene Construction (2012, background): “They are attB1 and attB2, attP1 and attP2, attL1 and attL2, and attR1 and attR2; a recombination reaction can occur only in the combinations of attB1 and attP1, attB2 and attP2, attL1 and attR1, or attL2 and attR2, since recombination strictly depends on att sequences (Hartley et al., 2000; Walhout ”
• Gateway vectors for plant transformation (2009, methodology): “The Gateway cloning method uses these att sites and clonases for construction of plasmid in vitro (Hartley et al. 2000, Walhout et al. 2000).”