Improvement of Bacterial Blight Resistance of ‘Minghui 63’, an Elite Restorer Line of Hybrid Rice, by Molecular Marker‐Assisted Selection
By Sheng Chen, X. Lin, C. Xu, Qifa Zhang. 2000
226 citations recorded by the source corpus.
'Minghui 63' is a restorer line widely used in hybrid rice production in China. However, this line has become increasingly susceptible to bacterial blight (BB), resulting in a rapid decline of its use in rice production. The objective of this study was to improve the BB resistance of Minghui 63 by introgressing Xa21, a broad-spectrum BB resistance gene, into Minghui 63 by molecular marker-assisted selection (MAS). A polymerase chain reaction (PCR)-based MAS system was established consisting of a marker that is a part of Xa21, a marker located at 0.8 centimorgans (cM) from the Xa21 locus on one side, and a marker at 3.0 cM from the gene on the other side. A total of 128 restriction fragment length polymorphism (RFLP) markers, evenly distributed on the 12 chromosomes, were used to recover the genetic background of Minghui 63. The resulting improved version of Minghui 63, or Minghui 63(Xa21)', was exactly the same as the original except for a fragment of less than 3.8 cM in length surrounding the Xa21 locus. Both Minghui 63(Xa21) and its hybrid with 'Zhenshan 97A' referred to as 'Shanyou 63(Xa21)' showed the same spectrum of BB resistance as the donor parent. Field examination of a number of agronomic traits showed that Minghui 63(Xa21) and Shanyou 63(Xa21) were identical to Minghui 63 and Shanyou 63, when there was no disease stress. Under heavily diseased conditions, Minghui 63(Xa21) showed significantly higher grain weight and spikelet fertility than Minghui 63, and Shanyou 63(Xa21) was significantly higher than Shanyou 63 in grains per panicle, grain weight, and yield.
Subfields: Plant Science
View Paper: https://www.semanticscholar.org/paper/2adb7a554b341413e0e97c92cccbc3d4e75c25e3
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Citations in the corpus
- Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid (2010, methodology): “…performance of the original Pusa RH10 with resistance to BB. Marker-assisted selection for transfer of BB resistance genes has been successfully utilized in several studies (Huang et al. 1997; Sanchez et al. 2000; Chen et al. 2000, 2001; Singh et al. 2001; Narayanan et al. 2004; Perez et al. 2008).”
- Genetic improvement of C4 grasses as cellulosic biofuel feedstocks (2009, background): “For example, Xa21-mediated disease resistance (Chen et al. 2000) and Bph1 and Bph2-mediated insect resistance (Sharma et al.”
- Improving bacterial blight resistance of '6078', an elite restorer line of hybrid rice, by molecular marker-assisted selection (2001, methodology): “The advantage and feasibility of practising background selection in the BC1F1 generation was discussed previously (Chen et al. 2000), and it was suggested that background selection in BC1F1 among individuals with recombinations on either side of the targeted gene be conducted.”
- Breeding crops for reduced-tillage management in the intensive, rice–wheat systems of South Asia (2006, methodology): “The bacterial blight resistance gene Xa21 (Chen et al. 2000) was backcrossed into rice cultivars in China and India, as well as into elite IRRI lines (Chen et al. 2000; Sanchez et al. 2000; Singh et al. 2001).”
- Implementation of molecular markers for quantitative traits in breeding programs - challenges and opportunities (2004, background): “Xa21 is a highly desirable allele because it confers resistance to most known races of the pathogen ( Chen et al. 2000; Singh et al. 2001), but the global scale of the deployment of this resistance gene increases the selection pressure for virulence in pathogen populations, which could lead to rapi”
- Rapid and high-precision marker assisted backcrossing to introgress the SUB1 QTL into BR11, the rainfed lowland rice mega variety of Bangladesh (2011, methodology): “This approach has been used with great success for ‘enhancing’ rice varieties for traits such as bacterial blight resistance gene XA21; (Chen et al. 2000), the waxy locus for grain quality (Zhou et al.”
- Pyramiding of Xa7 and Xa21 for the improvement of disease resistance to bacterial blight in hybrid rice (2006, methodology): “A plant was classified from its average lesion length as resistant ( 9.0 cm) ( Chen et al. 2000 ).”