Cryopreservation of fish sperm: applications and perspectives
By E. Cabrita, Carmen Sarasquete, S. Martínez-Páramo, Vanesa Robles, J. Beirão, S. Pérez-Cerezales, M. Herráez. 2010
365 citations recorded by the source corpus.
Summary Cryopreservation is of interest not only for fish farming but also for the conservation and genetic improvement of resources. This technique has been well established in some freshwater fish species mainly, salmonid, sturgeons and carps, however, only in the last decade research was focused in marine fish species. The benefits of sperm cryopreservation include: (i) synchronization of gamete availability of both sexes, (ii) sperm economy; (iii) simplification of broodstock management, (iv) transport of gametes from different fish farms, and (v) germplasm storage for genetic selection programs or conservation of species. These issues would certainly benefit the aquaculture industry. The tremendous impact that biotechnology is having in aquaculture has been particularly obvious in recent years. Several species are being used as research models not only for aquaculture development applications but also for medical research. Sperm cryopreservation can give an important contribution in the germ storage of all transgenic lines. However, in all applications in fish sperm, cryopreservation needs to overcome a lack in standardization of methodologies and procedures, a correct assay of seminal quality and the development of tools to characterize cryoinjury. Many efforts have recently been made in the study of DNA using different approaches such as the comet assay (single cell gel electrophoresis), TUNEL (terminal deoxynucleotidyl transferase-nick-end-labelling), SCSA (sperm chromatin structure assay) and the analysis of specific DNA sequences using RT-PCR, since DNA damage may impair fertility or embryo development. Cryopreservation of gametes would certainly benefit from a higher concern on male improvement, basically through nutrition or selection of resistant stocks (e.g. stress resistant individuals or highly adapted to captivity) producing gametes of higher quality. There is a huge window of opportunities for improve the resistance of cells to cryopreservation through diet supplementation of certain compounds such as amino acids (taurine and hypotaurine), vitamins (Vit. E and C) and lipids or through a direct supplementation of the extender media. An equilibrium of those compounds will improve spermatozoa and seminal plasma composition protecting cells against oxidative stress (lipid peroxidation, protein oxidation, DNA fragmentation, enzyme protection) that is gaining each day more importance in cryodamage research.
Subfields: Genetics, Physiology, Reproductive Medicine
View Paper: https://www.semanticscholar.org/paper/2a9e80cd750c17b40aa161588a3f1d87738d266f
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Citations in the corpus
- Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality (2017, background): “These parameters include the presence of females (Cabrita et al., 2010), hierarchical position of dominant versus subordinate fish (Leach and Montgomerie, 2000; Rudolfsen et al.”
- Cryopreservation of rainbow trout semen using a glucose-methanol extender (2014, background): “%) of post-thaw motility of rainbow trout semen was also observed by Cabrita et al. (2001, 2010), Tekin et al. (2003), and Ekici et al. (2012), but fertilization rates in these studies were lower than that of fresh semen.”
- Fish sperm motility assessment as a tool for aquaculture research: a historical approach (2019, background): “Although some previous manuscripts have 252 reviewed fish sperm cryopreservation subject (Suquet et al. 2000; Kopeika et al. 2007; 253 Cabrita et al. 2010; Asturiano et al. 2017; Martínez-Páramo et al. 2017), Table 2 254 summarizes the best results reached on fish sperm cryopreservation on the most…”