G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

By I. Macaulay, W. Haerty, Parveen Kumar, Yang I Li, T. X. Hu, Mabel J Teng, Mubeen Goolam, Nathalie Saurat, P. Coupland, Lesley M Shirley and 10 more. Nature Methods, 2015

720 citations recorded by the source corpus.

The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/1620369c50bf7eb4f84646ef36de48a78a36385d

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Citations in the corpus

From Big Data to Precision Medicine (2019, positive): “It is now possible to measure concurrent transcriptomes and genetics [so-called G&T seq (28)] or epigenetic modiﬁcations (29) on a single cell.”
Interpretable dimensionality reduction of single cell transcriptome data with deep generative models (2017, neutral): “The number of layers was ﬁ xed at ten for both the variational in ﬂ uence neural networks and the model neural networks; the number of units in each layer was set to 8, 16, 32, 64, and 128.”
Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq (2017, background): “Although genetic mutations may be used for such classification, mutation calling from scRNA-seq has limited sensitivity and specificity, and combined single-cell DNA and RNA profiling is not yet scalable to thousands of cells (9, 10).”
Defining cell types and states with single-cell genomics (2015, background): “Nevertheless, two recent studies demonstrated how single cells can be genotyped and profiled for global gene expression simultaneously (Dey et al. 2015;Macaulay et al. 2015).”
The technological landscape and applications of single-cell multi-omics (2023, methodology): “The next year, genome and transcriptome sequencing (G&T-seq) 21 and gDNA–mRNA sequencing (DR-seq) 22 were reported.”