Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses

By Yonit Lavin, Soma Kobayashi, A. Leader, Naama Elefant, Camille Bigenwald, R. Remark, Robert Sweeney, C. D. Becker, Jacob H. Levine, K. Meinhof and 18 more. Cell, 2017

1,065 citations recorded by the source corpus.

Summary To guide the design of immunotherapy strategies for patients with early stage lung tumors, we developed a multiscale immune profiling strategy to map the immune landscape of early lung adenocarcinoma lesions to search for tumor-driven immune changes. Utilizing a barcoding method that allows a simultaneous single cell analysis of the tumor, non-involved lung and blood cells together with multiplex tissue imaging to assess spatial cell distribution, we provide a detailed immune cell atlas of early lung tumors. We show that stage I lung adenocarcinoma lesions already harbor significantly altered T cell and NK cell compartments. Moreover, we identified changes in tumor infiltrating myeloid cell (TIM) subsets that likely compromise anti-tumor T cell immunity. Paired single cell analyses thus offer valuable knowledge of tumor-driven immune changes, providing a powerful tool for the rational design of immune therapies.

Subfields: Immunology, Molecular Biology, Oncology

View Paper: https://www.semanticscholar.org/paper/2a7b9a61aef2d1cf94f3fbc5f9ca3c1a9c7f88b9

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Citations in the corpus

Single-cell analysis reveals fibroblast clusters linked to immunotherapy resistance in cancer. (2020, background): “21 these studies being focused on immune cell heterogeneity (31,45-51).”
Organoid modeling of the tumor immune microenvironment (2018, methodology): “These can be confounded by antibody specificity, tumor PD-L2 expression, PD-L1 expression on multiple TME cell types (Topalian et al., 2016), and significant false-positive and falsenegatives (Carbognin et al., 2015; Garon et al., 2015).”
Dysfunctional CD8+ T cells form a proliferative, dynamically regulated compartment within human melanoma (2018, positive): “The presence of various myeloid cell populations has previously been suggested as a potential modifier of T cell activity in tumors (Binnewies et al., 2018; Lavin et al., 2017; Salmon et al., 2016).”