OMIP‐44: 28‐Color Immunophenotyping of the Human Dendritic Cell Compartment

By F. Mair, Martin Prlic. 2018

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This work describes the first 30-parameter immunophenotyping of the human dendritic cell (DC) compartment using fluorescent-based flow cytometry. The optimized panel allows for simultaneous detection of 21 myeloid-centric markers distinguishing all canonical DC subsets, with parallel enumeration of monocytes, T and B cells as well as NK cells. Thus, this panel will be useful for extensive phenotyping of immune cells from a variety of human samples limited in size.

Subfields: Immunology, Radiology, Nuclear Medicine and Imaging