An efficient protocol for the regeneration of whole plants of chickpea (Cicer arietinum L.) by using axillary meristem explants derived from in vitro-germinated seedlings

By B. Jayanand, G. Sudarsanam, K. Sharma. In Vitro Cellular & Developmental Biology - Plant, 2003

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Impact of Genomic Technologies on Chickpea Breeding Strategies (2012, background): “Efficient transformation and regeneration protocols are now available for chickpea [18], which have made it possible to introduce any desired gene from any source into chickpea.”
Chickpea molecular breeding: New tools and concepts (2006, background): “ICRISAT has developed an efficient transformation and regeneration system (Jayanand et al., 2003), and developed transgenic plants with a dehydration responsive element (DRE) construct, where the expression of the DREB1A cDNA is driven by a droughtresponsive rd29A promoter (ICRISAT, 2003).”
Genetic engineering of chickpea (Cicer arietinum L.) with the P5CSF129A gene for osmoregulation with implications on drought tolerance (2009, methodology): “The plants were rooted and hardened as described previously ( Jayanand et al. 2003 ) and transferred to a containment greenhouse with 24/18C day/night temperatures where they were allowed to grow until maturity and subsequent progression of generations (T1, T2, and so on).”