Identification of Photobionts from the lichen family Physciaceae using algal-specific ITS rDNA sequencing

By Gert Helms, T. Friedl, G. Rambold, H. Mayrhofer. The Lichenologist, 2001

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Revisiting photobiont diversity in the lichen family Verrucariaceae (Ascomycota) (2011, background): “…symbionts, such as algae growing on the lichen thallus surface or in cracks or cavities within the lichen (accessory algae), and it may therefore be difficult to distinguish the main symbiotic algal component of a lichen thallus from opportunistic cohabitants (Helms et al., 2001; Honegger, 2009).”
Assessing host specialization in symbiotic cyanobacteria associated with four closely related species of the lichen fungus Peltigera (2005, methodology): “Sequences of 16S and trnL were aligned with the aid of the secondary structure information ( Gutell, 1993; Paulsrud & Lindblad, 1998; Paulsrud et al., 1998; Costa et al., 2002).”
High photobiont diversity associated with the euryoecious lichen‐forming ascomycete Lecanora rupicola (Lecanoraceae, Ascomycota) (2006, background): “Recently, phylogenetic hypotheses showed that these characters do not necessarily reflect monophyletic lineages in Trebouxia (Helms et al., 2001), but only in a few cases have molecular data contributed to a reevaluation of the species concept so far.”
A microbiotic survey of lichen-associated bacteria reveals a new lineage from the Rhizobiales (2009, background): “hypothesis that some lineages of bacteria may be specific to the lichen thallus, similar to certain green-algal and cyanobacterial lineages ( Helms et al., 2001; Miadlikowska et al., 2006; Lucking et al., 2009).”