Highly efficient vitrification method for cryopreservation of human oocytes.

By M. Kuwayama, G. Vajta, O. Kato, S. Leibo. Reproductive Biomedicine Online, 2005

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The mammalian ovary from genesis to revelation. (2009, background): “(719) demonstrated higher oocyte survival, fertilization, and pregnancy outcome when a slightly lower concentration of ethylene glycol (5.”
Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. (2007, background): “Twentynine embryo transfers with a mean number of 2.2 embryos per transfer on days 2 and 5 resulted in 12 initial pregnancies (pregnancy rate 41%) [ 48 ] and eventually 11 healthy babies were born from 10 deliveries.”
American Society of Clinical Oncology recommendations on fertility preservation in cancer patients. (2006, background): “There have been approximately 120 deliveries with this approach,(83) and efforts to improve the efficiency of cryopreservation may increase success rates.(84,85) Further research is needed to delineate the current success rates and safety, as well as to improve the efficiency Table 5.”
Oocyte, embryo and blastocyst cryopreservation in ART: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance (2016, methodology): “When restricting the analysis of observational studies to those including only blastocyst stage embryos (Kuwayama et al., 2005a,b; Stehlik et al., 2005; Liebermann and Tucker, 2006), there was no statistically significant difference in CPR per cycle or CPR per transfer with vitrification compared…”