A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies

By P. Borden, Peng Zhang, Amol V. Shivange, J. Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, Ondrej Novák, Masashi Tanimoto and 31 more. bioRxiv, 2020

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Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all animals.

Subfields: Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/287681757f82cb60b36f5b1caa10de9fc7761cbb