Automated unrestricted multigene recombineering for multiprotein complex production
By C. Bieniossek, Yan Nie, D. Frey, N. Olieric, C. Schaffitzel, I. Collinson, C. Romier, Philipp Berger, T. Richmond, M. Steinmetz and 1 more. 2009
108 citations recorded by the source corpus.
Subfields: Molecular Biology, Radiology, Nuclear Medicine and Imaging
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In Molecular Biology
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- In silico protein interaction screening uncovers DONSON’s role in replication initiation (2023, methodology): “S13 was generated using the Acembl/MultiCol system ( 55 ) by first cloning the PSF1, PSF2, and SLD5 subunits of Xenopus laevis GINS into a pDC donor plasmid and PSF3 (with a C-terminal His6 tag connected through a LPETG tag and 10-aa linker) into a pACE2 acceptor plasmid.”