Plant age and genotype impact the progression of bacterial community succession in the Arabidopsis rhizosphere

By S. Micallef, S. Channer, M. Shiaris, Adán Colón-Carmona. 2009

268 citations recorded by the source corpus.

The rhizosphere is strongly influenced by plant-derived phytochemicals exuded by roots and plant species exert a major selective force for bacteria colonizing the root-soil interface. We have previously shown that rhizobacterial recruitment is tightly regulated by plant genetics, by showing that natural variants of Arabidopsis thaliana support genotype-specific rhizobacterial communities while also releasing a unique blend of exudates at six weeks post-germination. To further understand how exudate release is controlled by plants, changes in rhizobacterial assemblages of two Arabidopsis accessions, Cvi and Ler where monitored throughout the plants’ life cycle. Denaturing gradient gel electrophoresis (DGGE) fingerprints revealed that bacterial communities respond to plant derived factors immediately upon germination in an accession-specific manner. Rhizobacterial succession progresses differently in the two accessions in a reproducible manner. However, as plants age, rhizobacterial and control bulk soil communities converge, indicative of an attenuated rhizosphere effect, which coincides with the expected slow down in the active release of root exudates as plants reach the end of their life cycle. These data strongly suggests that exudation changes during plant development are highly genotype-specific, possibly reflecting the unique, local co-evolutionary communication processes that developed between Arabidopsis accessions and their indigenous microbiota.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/27bc8578abb4b01406b5a9b1af699de6aa669249

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Citations in the corpus

Abiotic Stress Responses and Microbe-Mediated Mitigation in Plants: The Omics Strategies (2017, background): “…February 2017 | Volume 8 | Article 172 This observation correlates with the reports pertaining to the identification of various signal molecules secreted by plants to attract and induce important biochemical pathways in colonizing microbial population (Zhang and Cheng, 2006; Micallef et al., 2009).”
Rhizosphere microbiome assemblage is affected by plant development (2013, background): “These results are in agreement with previous reports as the Arabidopsis rhizosphere microbial communities after the bolting stage were not distinct (Micallef et al., 2009a; Lundberg et al., 2012).”
Root Exudation: The Ecological Driver of Hydrocarbon Rhizoremediation (2016, background): “The structure of the rhizosphere microbial community depends on soil type [75–78], temporal changes (seasons) and environmental factors [79,80], plant species and genotype [75,81–85], plant development stage [85–87] and signaling of plant hormones [88].”
Impact of plant domestication on rhizosphere microbiome assembly and functions (2015, background): “A study with eight Arabidopsis thaliana accessions revealed that each accession released a particular set of exudate compounds and that each accession had a distinct rhizobacterial community composition based on RISA and 16S-TRFLP analyses (Micallef et al. 2009b).”
Plant nitrogen uptake drives rhizosphere bacterial community assembly during plant growth (2015, background): “Mounting evidence that rhizosphere bacterial community composition changes throughout plant developmental stages (Houlden et al., 2008; Micallef et al., 2009; Ziegler et al., 2013) suggests that plants do have an important role in structuring soil bacterial communities throughout the growing season…”