Spatial light interference microscopy (SLIM)
By Zhuo Wang, L. Millet, Mustafa Mir, Huafeng Ding, S. Unarunotai, John A. Rogers, M. Gillette, Gabriel Popescu. IEEE Photonic Society 24th Annual Meeting, 2011
626 citations recorded by the source corpus.
Spatial light interference microscopy (SLIM) is a new optical microscopy technique, capable of measuring nanoscale structures and dynamics in live cells via interferometry. SLIM combines two classic ideas in light imaging: Zernike's phase contrast microscopy, which renders high contrast intensity images of transparent specimens, and Gabor's holography, where the phase information from the object is recorded. Thus, SLIM reveals the intrinsic contrast of cell structures and, in addition, renders quantitative optical path-length maps across the sample. The resulting topographic accuracy is comparable to that of atomic force microscopy, while the acquisition speed is 1,000 times higher. We illustrate the novel insight into cell dynamics via SLIM by experiments on primary cell cultures from the rat brain. SLIM is implemented as an add-on module to an existing phase contrast microscope, which may prove instrumental in impacting the light microscopy field at a large scale.
Subfields: Atomic and Molecular Physics, and Optics, Biomedical Engineering, Biophysics
View Paper: https://www.semanticscholar.org/paper/277c68d773932fb2e27b845b1267e23ac9c81b98
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In Atomic and Molecular Physics, and Optics
In Biomedical Engineering
Citations in the corpus
- Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications (2013, background): “light interference microscopy (SLIM) [18] have been introduced.”
- PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning (2018, methodology): “For the generator deep network, we defined the loss function as: { } { } generator 1 label input input input 2 , ( ) TV ( ) (1 ( ( ))) L z G x G x D G x λ α = + × + × − l (4)”