Fungal Secondary Metabolism

By N. Keller, G. Turner. Methods in Molecular Biology, 2012

115 citations recorded by the source corpus.

High-throughput sequencing (HTS) has quickly become a valuable tool for comparative genetics and genomics and is now regularly carried out in laboratories that are not connected to large sequencing centers. Here we describe an updated version of our protocol for constructing singleand paired-end Illumina sequencing libraries, beginning with puri fi ed genomic DNA. The present protocol can also be used for “multiplexing,” i.e. the analysis of several samples in a single fl owcell lane by generating “barcoded” or “indexed” Illumina sequencing libraries in a way that is independent from Illumina-supported methods. To analyze sequencing results, we suggest several independent approaches but end users should be aware that this is a quickly evolving fi eld and that currently many alignment (or “mapping”) and counting algorithms are being developed and tested.

Subfields: Molecular Biology, Pharmacology

View Paper: https://www.semanticscholar.org/paper/270c5abd3484a3d5a62a2ebb21f51c057c289ec7