Illumina sequencing library preparation for highly multiplexed target capture and sequencing.

By M. Meyer, Martin Kircher. Cold Spring Harbor Protocols, 2010

2,252 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/2638f3a8c9bedcdc457842797d699918f971306c

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Citations in the corpus

Ancient DNA (2019, background): “From these environments, reasonably well-preserved specimens with low levels of contamination have yielded exceptional amounts of data when next-generation sequencing techniques are applied [29].”
Not All Sequence Tags Are Created Equal: Designing and Validating Sequence Identification Tags Robust to Indels (2012, background): “Several freely available sets of edit metric sequence tags [41] or edit metric sets output by tag design programs [29] contained pairwise comparisons below the minimum expected edit distance (Figures S3, S4).”
Integrative and comparative genomic analysis of HPV-positive and HPV-negative head and neck squamous cell carcinomas (2014, methodology): “Sequencing data generation and analysis DNA sequencing libraries were prepared following published protocols (19) and enriched using custom capture reagents [Agilent; Nimblegen (validation)].”
Double indexing overcomes inaccuracies in multiplex sequencing on the Illumina platform (2011, methodology): “An amount of 5 ml of the eluates were used as template for the second round of amplification, which was performed in 100ml reactions containing 1 Phusion High Fidelity Mastermix (NEB) and the primers IS5 and IS6 (9) at a concentration of 400 nM each.”
High‐throughput DNA sequencing – concepts and limitations (2010, background): “These molecules can then be sequenced in the same region/channel, and later separated (computationally) based on their barcode sequence [70–73].”
Anchored hybrid enrichment for massively high-throughput phylogenomics. (2012, background): “Two-fold degeneracy of indexes is necessary to avoid an excessive number of misidentified reads (Meyer and Kircher 2010).”
Resolving the psyllid tree of life: phylogenomic analyses of the superfamily Psylloidea (Hemiptera) (2018, methodology): “Library preparation and indexing were performed following a protocol modified from Meyer & Kircher (2010) (described in Lemmon et al., 2012).”
The genetic history of Ice Age Europe (2016, neutral): “46 33,760-32,840 Direct-UF (6) Unassigned Temporal 3.”