Mitochondrial membrane potential probes and the proton gradient: a practical usage guide.
By S. Perry, J. Norman, Justin Barbieri, E. Brown, H. Gelbard. BioTechniques, 2011
1,155 citations recorded by the source corpus.
Fluorescent probes for monitoring mitochondrial membrane potential are frequently used for assessing mitochondrial function, particularly in the context of cell fate determination in biological and biomedical research. However, valid interpretation of results obtained with such probes requires careful consideration of numerous controls, as well as possible effects of non-protonic charges on dye behavior. In this context, we provide an overview of some of the important technical considerations, controls, and parallel complementary assays that can be employed to help ensure appropriate interpretation of results, thus providing a practical usage guide for monitoring mitochondrial membrane potentials with cationic probes. In total, this review will help illustrate both the strengths and potential pitfalls of common mitochondrial membrane potential dyes, and highlight best-usage approaches for their efficacious application in life sciences research.
Subfields: Clinical Biochemistry, Molecular Biology, Spectroscopy
View Paper: https://www.semanticscholar.org/paper/2636e0faa659c327315660c2f290f058d2e7733a
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Citations in the corpus
- Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe (2019, positive): “Fluorescent dye accumulation in mitochondria can be optically detected by flow cytometry, fluorescent microscopy, confocal microscopy, and through the use of a fluorescence plate reader (Perry et al., 2011).”