Conserved properties of dentate gyrus neurogenesis across postnatal development revealed by single-cell RNA sequencing

By Hannah Hochgerner, Amit Zeisel, P. Lönnerberg, S. Linnarsson. 2018

434 citations recorded by the source corpus.

The dentate gyrus of the hippocampus is a brain region in which neurogenesis persists into adulthood; however, the relationship between developmental and adult dentate gyrus neurogenesis has not been examined in detail. Here we used single-cell RNA sequencing to reveal the molecular dynamics and diversity of dentate gyrus cell types in perinatal, juvenile, and adult mice. We found distinct quiescent and proliferating progenitor cell types, linked by transient intermediate states to neuroblast stages and fully mature granule cells. We observed shifts in the molecular identity of quiescent and proliferating radial glia and granule cells during the postnatal period that were then maintained through adult stages. In contrast, intermediate progenitor cells, neuroblasts, and immature granule cells were nearly indistinguishable at all ages. These findings demonstrate the fundamental similarity of postnatal and adult neurogenesis in the hippocampus and pinpoint the early postnatal transformation of radial glia from embryonic progenitors to adult quiescent stem cells.Using single-cell RNA-seq, the authors show that early developmental neurogenesis in the dentate gyrus of the hippocampus is largely conserved in the adult, but with a perinatal transformation of stem cells to an adult type.

Subfields: Cancer Research, Developmental Neuroscience, Molecular Biology

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Citations in the corpus

openTSNE: a modular Python library for t-SNE dimensionality reduction and embedding (2019, methodology): “For instance, in Figure 7, we construct a reference embedding (using the same code snippet as above) using labeled data from Hochgerner et al. (2018) containing gene-expression profiles of cells from the mouse brain.”
A Single-Cell Atlas of Cell Types, States, and Other Transcriptional Patterns from Nine Regions of the Adult Mouse Brain (2018, background): “…of which two are native to the ventricle – the choroid plexus and ependymal cells, which had been sampled from ventricular tissue proximal to the hippocampal dissections, and an additional cell class undergoing adult neurogenesis (Habib et al., 2016; Hochgerner et al., 2018; Ming and Song, 2011).”
Mapping Transcriptomic Vector Fields of Single Cells (2022, background): “2017), dentate gyrus (Hochgerner et al. 2018), and fetal forebrain (La Manno et al.”
Molecular Architecture of the Mouse Nervous System (2018, methodology): “In the subgranular zone of the dentate gyrus, radial glia-like cells (RGDG), neuroblasts (NBDG) and immature granule cells (DGNBL1 and DGNBL2) would give rise to mature granule cells (DGGC), as recently described in detail (Hochgerner et al., 2018).”