Revisiting Cardiac Cellular Composition
By Alexander R. Pinto, A. Ilinykh, Malina J Ivey, Jill T. Kuwabara, M. D’Antoni, R. Debuque, Anjana Chandran, Lina Wang, Komal Arora, Nadia A. Rosenthal and 1 more. Circulation Research, 2015
1,202 citations recorded by the source corpus.
Rationale Accurate knowledge of the cellular composition of the heart is essential to fully understand the changes that occur during pathogenesis and to devise strategies for tissue engineering and regeneration. Objective To examine the relative frequency of cardiac endothelial cells, hematopoietic-derived cells and fibroblasts in the mouse and human heart. Methods and Results Using a combination of genetic tools and cellular markers, we examined the occurrence of the most prominent cell types in the adult mouse heart. Immunohistochemistry revealed that endothelial cells constitute over 60%, hematopoietic-derived cells 5–10%, and fibroblasts under 20% of the non-myocytes in the heart. A refined cell isolation protocol and an improved flow cytometry approach provided an independent means of determining the relative abundance of non-myocytes. High dimensional analysis and unsupervised clustering of cell populations confirmed that endothelial cells are the most abundant cell population. Interestingly, fibroblast numbers are smaller than previously estimated, and two commonly assigned fibroblast markers, Sca-1 and CD90, underrepresent fibroblast numbers. We also describe an alternative fibroblast surface marker that more accurately identifies the resident cardiac fibroblast population. Conclusions This new perspective on the abundance of different cell types in the heart demonstrates that fibroblasts comprise a relatively minor population. By contrast, endothelial cells constitute the majority of non-cardiomyocytes and are likely to play a greater role in physiologic function and response to injury than previously appreciated.
Subfields: Cardiology and Cardiovascular Medicine, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/245d29482f61b38e53dcf9331ef7577ccd495bd0
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In Cardiology and Cardiovascular Medicine
In Molecular Biology
Citations in the corpus
- Cardiac fibrosis in myocardial infarction—from repair and remodeling to regeneration (2016, background): “A recent report by Pinto et al. (2015), however, challenges this view by showing that endothelial cells and cardiomyocytes are the most abundant cell types in adult murine and human hearts, whereas fibroblasts are the third prevalent cell type in cell numbers (Fig.”
- The Biological Basis for Cardiac Repair After Myocardial Infarction: From Inflammation to Fibrosis. (2016, background): “The heart is a highly vascular organ; in adult mammals, endothelial cells are the most abundant noncardiomyocytes.(133)”
- Cardiac fibrosis. (2020, background): “Vascular endothelial cells are the most abundant noncardiomyocytes in adult mouse hearts, reflecting the rich microvasculature of the myocardium.(9) Smaller populations of immune cells (macrophages, mast cells, and dendritic cells), and vascular mural cells (smooth muscle cells and pericytes) are al”