Sampling, preservation, enumeration and biomass of marine protozooplankton

By D. J. Gifford, David A. Caron. 2000

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The fate of biogenic iron during a phytoplankton bloom induced by natural fertilisation: Impact of copepod grazing (2008, background): “Moreover, screening, filtration and recovering of the organisms from the filter is known to damage soft cells such as nanoflagellates and ciliates (Gifford, 1985; Gifford and Caron, 2000).”
Feeding by ciliates on two harmful algal bloom species, Prymnesium parvum and Prorocentrum minimum (2003, methodology): “Af ter 3–5 days, the ciliates were preserved using 10% acid lugols solution and counted using inverted light microscopy using methods described by Gifford and Caron (2000).”
The trophic roles of microzooplankton in marine systems (2008, background): “In the era of molecular biology, we do not even have a trustworthy way of preserving samples without losing a significant (and unknown) number of protists (Klein Breteler, 1985; Stoecker et al., 1994; Gifford and Caron, 2000; Broglio et al., 2004).”
Characteristics, distribution and persistence of thin layers over a 48 hour period (2003, methodology): “Microplankton (20 to 200 µm) samples were preserved with 10% (v/v) acid Lugol’s solution and 100 ml aliquot samples were processed using an inverted microscope at 200× magnification (Gifford & Caron 2000).”