Effect of plant growth regulators on the cellular growth and levels of intracellular protein, starch and polyamines in embryogenic suspension cultures of Pinus taeda

By V. Silveira, Eny Iochevet Segal Floh, W. Handro, Miguel Pedro Guerra. Plant Cell Tissue and Organ Culture, 2004

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Polyamine effects on the endogenous polyamine contents, nitric oxide release, growth and differentiation of embryogenic suspension cultures of Araucaria angustifolia (Bert.) O. Ktze. (2006, methodology): “Samples were ground in 1.6 ml of a 5% (v/v) perchloric acid solution, and free PAs were extracted, dansylated and identified by reverse phase HPLC according to Silveira et al. [ 5 ].”
Polyamine effects on growth and endogenous hormones levels in Araucaria angustifolia embryogenic cultures (2007, methodology): “However, in suspension cultures of Pinus taeda (Silveira et al. 2004a) and the callus of Nicotiana tabacum (Rastogi and Davies 2000), high levels of endogenous Put were associated with cellular growth reduction.”