From fixed to FRAP: measuring protein mobility and activity in living cells

By E. Reits, J. Neefjes. Nature Cell Biology, 2001

697 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Analysis of binding reactions by fluorescence recovery after photobleaching. (2004, background): “The past few years have witnessed a dramatic increase in the application of fluorescence recovery after photobleaching (i.e., FRAP; reviewed in Meyvis et al., 1999; White and Stelzer, 1999; Reits and Neefjes, 2001; Houtsmuller and Vermeulen, 2001).”
Anomalous transport in the crowded world of biological cells (2013, background): “research [212] as well as in living cells [214].”
Cell Biology of Cnidarian-Dinoflagellate Symbiosis (2012, background): “Imaging techniques such as fluorescence recovery after photobleaching (FRAP) (308), which follows the dynamic diffusion and movement of subcellular compartments and macromolecules, could be used in conjunction with numerous membrane trafficking probes to capture the dynamics Davy et al.”
A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation. (2015, positive): “Analysis of the recovery curves and the half-time recovery were carried out with the FIJI/ImageJ macro (http://fiji.sc/Analyze_FRAP_movies_with_a_Jython_script) (Reits and Neefjes, 2001; Sprague and McNally, 2005).”