Identification of gut-associated amylase, cellulase and protease-producing bacteria in three species of Indian major carps.

By A. Ray, Tanami Roy, Sabyasachi Mondal, E. Ringø. 2009

135 citations recorded by the source corpus.

Isolation and enumeration of amylase, cellulase and protease-producing autochthonous bacteria in the proximal intestine (PI) and distal intestine (DI) of three species of Indian major carps, catla (Catla catla), mrigal (Cirrhinus mrigala) and rohu (Labeo rohita), were investigated using the conventional culture-based technique. Population levels of amylolytic strains were the highest in the PI of catla and the lowest in the DI of rohu. The highest viable count of cellulase and protease-producing bacteria was recorded in the DI and PI of mrigal respectively. Among the bacteria isolated, 10 strains (five from PI and five from DI) were selected as potent enzyme producers according to a quantitative enzyme assay. The chosen strains were further identified by 16S rRNA gene sequence analysis. The five strains isolated from catla showed high similarity to Citrobacter sp. clone W2, Enterobacter sp. JA24, Bacillus coagulans strain TR, uncultured bacterial clone Hel3bc04 and Bacillus cereus strain UST2006-BC004. The four strains isolated from mrigal were most closely related to Bacillus sp. KCd2, uncultured bacterial clone Hel3bd09, B. cereus strain BU040901-020 and Citrobacter freundii strain YRL11, while the strain isolated from rohu probably belonged to Bacillus sp. GV.

Subfields: Biotechnology, Immunology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/22c5e855535a31ea67ad3535e4adac11fa2cd189

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In Immunology

1. S. Hoseinifar
2. E. Ringø
3. K. Ghosh
4. Vikash Kumar
5. Suvra Roy
6. M. Soltani
7. A. Ray
8. G. Banerjee

In Molecular Biology

1. E. Ringø
2. S. Hoseinifar
3. K. Ghosh
4. A. Ray
5. G. Banerjee

Citations in the corpus

• Genus bacillus, promising probiotics in aquaculture: Aquatic animal origin, bio-active components, bioremediation and efficacy in fish and shellfish (2019, background): “Ray et al. (2010) isolated amylase, cellulose, and protease-producing autochthonous bacteria in the PI and DI of three species of Indian major carps, catla, mrigal, and rohu, by cultivation and one of the strains from the PI of catla showed closely matched B. coagulans.”
• Combined effects of exogenous enzymes and probiotic on Nile tilapia (Oreochromis niloticus) growth, intestinal morphology and microbiome (2016, unknown): “…the ability of probiotic to produce fibre-degrading enzymes that may complement endogenous enzyme activity for digestion in fish (Roy et al., 2009, Ray et al., 2010, Ray et al., 2012) as well as the external exogenous enzyme capacity to increase the availability of suitable substrates for…”