Fluorescence microscopy

By Jeff William Lichtman, J. Conchello. Nature Methods, 2005

1,358 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/2161c7ef62f324d43d434181e7ab4b01e8ed7ea8

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Citations in the corpus

Fluorescence microscopy for visualization of soil microorganisms—a review (2004, background): “The key components of epifluorescence microscopy include an excitation light source, wavelength selection devices (a set of well-balanced filter combinations), objectives, and detectors (Ploem 1993; Herman 1998).”
Surface enhanced fluorescence (2008, background): “where EM represents all the EM relaxation processes and other are related to non-radiative relaxation processes induced by direct molecular interaction, essentially molecular collision [22].”
Immunofluorescence Microscopy (2008, methodology): “Originally published in CP Immunology UNIT 21.2 (Herman, 2002).”
Goals and practicalities of immunoblotting and immunohistochemistry: A guide for submission to the British Journal of Pharmacology (2018, background): “This is problematic when a sample is incubated with several fluorophores with overlapping excitation and emission profiles (Ploem, 1999; Bolte and Cordelieres, 2006; Rietdorf and Stelzer, 2006; Waters, 2009).”
What spatial light modulators can do for optical microscopy (2011, background): “The disadvantage is the fact that the preparation of the sample with fluorescence markers can also introduce undesired effects, such as photo-toxicity, photo-bleaching, or blinking [7, 8].”
Accuracy and precision in quantitative fluorescence microscopy (2009, methodology): “Bleed-through of one fluorophore’s emission through the filter set of another fluorophore can occur when a specimen is labeled with multiple fluorophores whose excitation and emission spectra overlap (Fig. 5; Ploem, 1999; Bolte and Cordelieres, 2006; Rietdorf and Stelzer, 2006).”
Histology image analysis for carcinoma detection and grading (2012, methodology): “Fluorescence microscopy: Fluorescence microscopy [52,71,94] is used to examine specimens using the absorption and subsequent re-radiation phenomena of fluorescence or phosphorescence.”
Visualization of image data from cells to organisms (2010, neutral): “Fluorescent markers make it possible to see particular structures with high contrast, either in fixed samples using immunostaining or in living cells with expressed GFP-tagged proteins 83 .”
Clarifying Tissue Clearing (2015, neutral): “Therefore, neither fluorescence (re-emission of lower-energy light; Lichtman and Conchello, 2005) nor ionization (removal of the electron from the atom or molecule) occurs.”