Aqueous Two Phase Systems for the Recovery of Biomolecules –A Review

By S. Raja, R. Murty, V. Thivaharan, V. Rajasekar, V. Ramesh. 2012

258 citations recorded by the source corpus.

Aqueous two phase system (ATPS) is a liquid – liquid extraction method which employs two aqueous phases having applications in the field of biotechnology for the separation and purification of biological materials such as proteins, enzymes, nucleic acids, virus, antibodies and cell organelles. This review discusses the basic principles of ATPS, the factors affecting partitioning, optimization by design of experiments and the recent applications like extractive fermentation, membrane supported liquid – liquid extraction and aqueous two phase floatation. A comparative study between ATPS and other conventional methods is also discussed. The emphasis is given to PEG/salt two-phase systems because of the low cost of the system.

Subfields: Filtration and Separation, Mechanical Engineering

View Paper: https://www.semanticscholar.org/paper/2140233d1458230ce70f4af4762853840680c276

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Citations in the corpus

• Aqueous Two-Phase Extraction Advances for Bioseparation (2013, background): “Recently, use of salts, like citrate (biodegradable) and ammonium carbonate (volatile) are favored because of their high selectivity, less pollution, biocompatibility and easy to scale-up [38,50].”
• Aqueous two-phase system (ATPS): an overview and advances in its applications (2016, methodology): “Finally, in all three screening designs (FFD, fFD and PBD) the magnitude of the significant factors is analyzed by using analysis of variance (ANOVA) [26, 44, 47].”